the roles of il-2, il-7, and il15 ligands in b cells development

abstract background b cells play an important role in the immune system due to production of the immunoglobulin and secreting several cytokines. it has been shown that b cells produced successfully in the presence of stem cell factor (scf) and flt3 ligand (flt3l). il2, il7, and il15 cytokines as  -common family have an essential cytokines may support either synergistically or in an additive manner to b cell proliferative activity. thus, the present study focused specifically on il2, il7, and il15 cytokines that may play a critical role in b cell proliferation in cord blood. in this study, we evaluated the generation of b cells from cd34+/- cord blood mononuclear cells by using il-2, il- 7, and il-15. material and methods cord blood mononuclear cells were cultured for 21 days in presence of different combination of il-2, il-7, and il-15. harvested cells were analyzed by flow cytometry at days 0, 7, 14 and, 21. results present study showed that b cell differentiation from cd34+ cord blood mononuclear cells was increased by using il-2 and il-7 at different time points however, il7 was more effective (p value < 0.0001). in contrast, il-15 didn’t increase significantly b cell expansion from cd34+ cord blood mononuclear cell. conclusion these findings showed that il-2 and il-7 significantly increased b cell generation from cord blood cd34+ cells probably this cytokines may be used in ex vivo generation of b cells from cord blood mononuclear cells.